Rainbow Railroad is a 4-star charity that is financially transparent. It has an A results reporting grade, which is above average. For every dollar donated, 69 cents are available to go to the cause. Its reserve funds (cash and investments) can cover just under two months of annual program costs, indicating a need for funding.

Founded in 2006, Rainbow Railroad helps at-risk LGBTQI+ people around the world reach safety, with a focus on relocating individuals to safer countries. Since its founding, the charity has helped over 2,553 people find safety. Rainbow Railroad has an office in Toronto and a US affiliate, Rainbow Railroad USA. The two charities operate under the same management and work closely together. While they report separate financial statements, they share an annual report.

Rainbow Railroad helps LGBTQI+ individuals by responding to requests for help made on the charity's website. Emergency Travel Support is Rainbow Railroad's core program. The charity helps relocate people that face immediate danger to a safer country through transportation and short-term support. Rainbow Railroad also offers financial aid, government sponsorship help, crisis response, and referrals to other organizations. The charity does not provide a breakdown of spending on its programs.  

In 2025, Rainbow Railroad supported people through a range of direct programs, including 7,128 people with Information Services and Referrals, 355 with Cash Assistance, 339 with Government Resettlement Pathways, 143 with Post-Relocation Support, 85 with Emergency Travel Support, and 27 with Refugee Leadership and Inclusion. The charity also supported people through funded partnerships, including 11,866 people through the Grassroots Mobilization Fund, 4,909 through the Crisis Response Fund, and 1,080 through the Community Access Fund.